GLD-1 is a protein expressed in the germline of the worm species C. elegans. I am doing a forward genetic screen to determine novel expression of GLD-1 and then mapping the mutation to its location in the genome. A forward genetic screen is comprised of exposing the worms to the mutagenic compound ENU and then observing GLD-1 expression in their offspring for several generations in order to determine if GLD-1 expression is varied. GLD-1 will be tagged with GFP, a fluorescent protein, so that it can be easily observed. If any novel GLD-1 phenotypes are observed, specifically if GLD-1 expression is extended past normal expression points in the germline, then these mutational alleles will be mapped to loci for further analysis.
GLD-1 is a protein expressed in the germline of the worm species C. elegans. I am doing a forward genetic screen to determine novel expression of GLD-1 and then mapping the mutation to its location in the genome. A forward genetic screen is comprised of exposing the worms to the mutagenic compound ENU and then observing GLD-1 expression in their offspring for several generations in order to determine if GLD-1 expression is varied. GLD-1 will be tagged with GFP, a fluorescent protein, so that it can be easily observed. If any novel GLD-1 phenotypes are observed, specifically if GLD-1 expression is extended past normal expression points in the germline, then these mutational alleles will be mapped to loci for further analysis.